2. Methods

 We will be carrying out the experiment in the 
Science Research lab. 


2.1.1  Dependent Variable:
Dependent Variable:
The amount of Oxygen collected in the test tube

2.1.2 Independent variable:

 Independent variable: 
7 LED lights (Red, Blue, Green, Purple[red+blue], Amber, Warm White, White) And no light.

2.1.3  Constant Variables:

  • Surrounding Temperature
  • Water Level
  • Type of water used
  • Amount of electricity lights are getting
  • Amount of Hydrogen Sodium Carbonate in water
  • Size of Apparatus
  • Opacity of Apparatus
  • Type of apparatus
  • Amount of plant
  • Type of plant
  • Distance of plant to light.

2.2 Equipment: 

  • 24 Similar pond weeds (Cabomba)
  • Sodium Hydrogen Carbonate
  • 4 Boxes with separators 
  • 8 Beakers
  • 8 Funnels
  • 8 Test tubes
  • Surrounding Humidity
  • Amount of time left in their conditions

2.3 Data collection Procedures:

The data collection process was conducted with 4 boxes containing each a beaker with the following set-up:
Fig 1. The setup that we will be using

                                     Fig 2.1
                                     Fig 2.2
                                     Fig 2.3
                                     Fig 2.4
The plant we used is Cabomba.
Sodium Hydrogen Carbonate solution is used to provide carbon dioxide for the plant to ensure they have sufficient carbon dioxide for photosynthesis. 

As the boxes are kept indoors, there is no specific day to check on the set-up. So the data collection are done on days that we chose.

Frequency of checking on the plants is every 24 hours.

Every 24 hours of said timings, the depth of the oxygen level of the set-ups would be measured with a ruler beside the test tubes, taken down and photographed. This helps us compare the set-ups to find out how coloured lights/wavelengths affects the plants' rate of photosynthesis and which coloured light/wavelength is the most efficient for photosynthesis.

1. Setup the apparatus in this manner.

2.  Place the setup in box for 24 hours

3. After 24 hours shake the test tube and funnel to allow the bubbles trapped under plant escape to the top.

4. Measure the amount of oxygen produced every 24 hours by taking the depth of oxygen in the test tube.

5. Repeat the experiment 3 times for more precise results.

The following wavelengths of the lights we chose are:
High Eff. Red
Incandescent White
Cool White
6500 + K
High Eff. Green
Super Blue
Purple (around the same as red)
Dark (no light)

2.4  Risk and Safety: Identify any potential risks and safety precautions to be taken.

Risk and Safety

1.As this experiment involves liquids, there is an imminent risk of spillage of the liquids, causing the experiment environment to be affected. (Make the boxes wet, soft, incapable of holding weight). To avoid this, apparatus should be used properly and with utmost care so as to not cause this to happen. Overfilling the beakers may cause spillage, thus it has to be avoided at all cost. As spillage of water may result, in (Risk 2) as well, it should be a point to be taken note of.

2.As the experiment involves electricity and water, there may be a risk of electric shocks if the electrical components are handled with wet hands or if they simply come into contact with water. To ensure this does not happen, apparatus have to be handled carefully and latex gloves are to be worn at all times while handling the apparatus to avoid shocks in case the water comes into contact with the electricity.

3.  As this experiment involves liquids, there is a risk of mosquito breeding, therefore we have to change the

water every few days or put a layer of oil over the water’s surface. As the risk of dengue being spread by aedes mosquito is evident, we must ensure that they do not breed mosquito in our apparatus to not spread the disease as well as not contaminate our experiment and cause inaccurate results.

4. After a few tests the LEDs are found to overheat quickly resulting in the silicon melting and sticking to other LEDs, the stick strips on the back also melted due to exposure to high heat. (Solution to combat this problem would be to make a rectangular clear plastic case that would be filled with water and the LEDs placed in it would ensure that the water would absorb the heat so lights would not overheat too quickly.) These lights were meant to be operated underwater so the risk of electric shock would be lowered.


2.5    Data Analysis: Describe the procedures you will use to analyze the data/results that answer research questions or hypothesis

Data Analysis

We will take the collected data of all three experiments and put results in a histogram to see the results clearly and that we can conclude which light is most effective

Example: (Note: Not actual results)
Fig 2.5

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